试管苗简化培养试验初探     

宋锋惠  史彦江  卡得尔 《西北林学院学报》2004,19(2):67-69

通过简化培养基成分,去除有机物,可抑制在长期继代培养中被茵类污染的试管苗;采用液体培养,可降低试管苗成本;用自然光代替日光灯,并结合黑暗培养,可减少设备投入,简化培养室条件,有利于工厂化育苗生产.  相似文献   

美国东部黑核桃不同家系幼树年生长节律研究     

宋锋惠  史彦江  卡得尔 《经济林研究》2006,24(1):56-59

通过对引进的3个家系美国东部黑核桃4-5年生幼树的年生长节律及物候分析比较,结果表明:(1)黑核桃春季萌动、抽梢生长较晚,生长期仅为80 d左右,高、径生长集中在5-7月;(2)为使新梢充分木质化,预防2次生长带来的早、晚霜危害,树木停止高生长后,及时有效控水;(3)同一种源不同家系间,高、径生长存在差异,引进种子苗木时,应考虑引进经过区域试验种源的优良家系。  相似文献   

猪流行性腹泻病毒S2基因B细胞表位嵌入型S-层蛋白在副干酪乳杆菌中的表达与鉴定     

白伟琴  卡楚拉  乌志勇  苗苗  塔娜  格日勒图 《中国畜牧兽医》2022,49(10):4004-4011

【目的】构建新型猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)乳酸菌活菌疫苗载体。【方法】应用DNA重组技术将嗜酸乳杆菌S-层蛋白(SLP)基因及PEDV S2基因B细胞表位(EpitopeS2)融合基因(SLP-EpitopeS2)克隆到乳酸杆菌表达载体pTRK892中,构建重组载体pTRK-SLP-EpitopeS2,通过电转化方法将重组质粒导入副干酪乳杆菌中,获得重组副干酪乳杆菌。分别用SDS-PAGE、Western blotting和免疫荧光试验(IFA)鉴定目的蛋白在副干酪乳杆菌中的表达。【结果】PCR结果显示,成功扩增出大小为1 400 bp的目的条带,与插入融合基因大小一致,双酶切结果出现大小分别为1 400和4 700 bp的2条带,基因测序结果显示无碱基缺失和突变等,从而确定重组质粒pTRK-SLP-EpitopeS2构建正确。SDS-PAGE、Western blotting结果显示,在48 ku处出现与理论值大小一致的目的蛋白条带,表明融合基因SLP-EpitopeS2在副干酪乳杆菌中得到有效表达。IFA结果显示,与对照组副干酪乳杆菌相比,重组副干酪乳杆菌均能被激发出特异性绿色荧光信号,与高浓度氯化锂(LiCl)洗脱下来的菌体膜蛋白样品补充鉴定试验结果相吻合。表明融合蛋白SLP-EpitopeS2可能在副干酪乳杆菌的菌体表面表达。【结论】成功构建了PEDV EpitopeS2及嗜酸乳杆菌SLP嵌入型融合表达载体pTRK-SLP-EpitopeS2,为乳杆菌活菌载体疫苗相关研究奠定了基础。  相似文献   

肉牛早期断奶试验研究     

周振勇  张杨  张金山  李红波  闫向民  杜玮  卡德尔 《中国牛业科学》2011,37(3):20-23

[目的]旨在研究早期断奶技术对不同品种肉用犊牛生长发育的影响,[方法]本试验在草原牧区应用犊牛代乳料产品开展了不同品种犊牛的早期断奶工作,[结果]表明:安杂牛、新褐杂牛、荷斯坦牛三组间日增重差异不显著(P>0.05),安杂组增重成本8.25元/kg,新褐杂增重成本组9.27元/kg,荷斯坦组增重成本9.62元/kg,但...  相似文献   

Pyloric outflow obstruction secondary to sclerosing encapsulating peritonitis in a dog     

KA Carroll  ML Wallace  TL Hill  JW Bartges  JL Ruby  AT Mulder II  JI Helmick 《Australian veterinary journal》2020,98(1-2):11-16

A 6-year-old, male neutered mixed breed dog was presented emergently with a three-week history of hyporexia, vomiting, diarrhoea and weight loss. Upon examination, the patient was dull, had generalised muscle atrophy, moderate abdominal pain and a mild amount of peritoneal effusion. A fluid-filled, distended, corrugated small bowel with marked gastroparesis and moderate peritoneal effusion was noted on abdominal ultrasonography. Endoscopy revealed hyperaemic and friable mucosa and a subjectively narrowed pylorus. Emergency exploratory celiotomy was performed due to worsening patient condition and revealed thick, diffuse, fibrous adhesions of the abdominal cavity. Based on these findings, sclerosing encapsulating peritonitis (SEP) was suspected. A large mass of omentum adjacent to the greater curvature of the stomach had caused a pyloric outflow obstruction. Adhesiolysis was attempted but was unsuccessful due to the friability of the small intestines. The dog was humanely euthanased under anaesthesia. A diagnosis of SEP was confirmed via necropsy. No underlying cause was identified. This is the first known case of a pyloric outflow obstruction secondary to SEP in a dog. Although rare, this condition should be considered as a differential for dogs with signs of a pyloric outflow obstruction with concurrent ascites and abdominal pain, hyporexia, vomiting and diarrhoea.  相似文献   

Detection and isolation of Toxoplasma gondii from fresh semen of naturally infected dogs in Southern Brazil          下载免费PDF全文

MO Koch  RR Weiss  AA Cruz  VT Soccol  KA Gonçalves  MAF Bertol  OC Beltrame  RL Dittrich 《Reproduction in domestic animals》2016,51(4):550-554

The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T. gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T. gondii in fresh semen samples using in vitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T. gondii was confirmed by in vitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa (TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T. gondii DNA sequences stored in the GenBank. It has been shown that T. gondii tachyzoites may be isolated in vitro from fresh semen samples of clinically healthy dogs seropositive for T. gondii.  相似文献   

Pregnancy-associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows     

KA El-Battawy  NM Sousa  O Szenci  JF Beckers 《Reproduction in domestic animals》2009,44(2):161-166

Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg prostraglandin (PG)F_{2 α} (Lutalyse^®) at a 11-day interval. Blood sampling was conducted nearly twice weekly. Results indicated that plasma PAG concentrations in non-pregnant buffaloes were low (<0.20 ng/ml) during the whole experimental period (day 28 to 103), while in pregnant animals plasma PAG levels increased from day 28 (4.48 ± 0.92 ng/ml) until day 41 (27.27 ± 6.74 ng/ml), remaining high (20.71 ± 9.20 ng/ml) until day 103. Progesterone levels were significantly (p < 0.0001) higher in pregnant (3.51–4.80 ng/ml) than in non-pregnant buffaloes (0.28–1.52 ng/ml). A significant difference (p < 0.0001) in plasma PAG concentrations between pregnant and non-pregnant animals starting at day 28 after breeding suggests that PAG-radioimmunoassay could be suitable for pregnancy diagnosis in buffaloes during this period. In conclusion, PAG test offers the advantages that it requires a single plasma sample for early pregnancy diagnosis as well as the accuracy of the test for the detection of pregnancy as early as day 28.  相似文献   

Bluetongue virus infection in sheep: haematological changes and detection by polymerase chain reaction   总被引：2，自引：0，他引：2  

KA McCOLL  AR GOULD 《Australian veterinary journal》1994,71(4):97-101

SUMMARY Eight sheep vaccinated with 10⁶ pfu of attenuated Australian bluetongue virus serotype 23 (BTV-free sheep were challenged with virulent BTV 23. There was little subsequent variation in the mean clinical score, or in the mean lymphocyte and platelet concentrations in the peripheral blood of the eight vaccinated sheep. There was a marked thrombocytopenia and lymphopenia in the naive sheep as the mean lymphocyte and platelet concentrations fell to a minimum at days 8 and 11 after inoculation, respectively. Similar changes were observed in three other naive sheep inoculated with field isolates of BTV 1, 9 or 23. BTV was detected by nested polymerase chain reaction in whole blood of these sheep between days 6 and 28, in mononuclear leukocytes between days 3 and 14, and in platelets between days 6 and 21.  相似文献   

Enterohaemorrhagic Escherichia coli: A new problem, an old group of organisms Inter faeces et urinam nascimur     

KA BETTELHEIM 《Australian veterinary journal》1996,73(1):20-26

All mammals are colonised by Escherichia coli generally at birth and these organisms become part of their intestinal flora for the rest of their lives. New types are acquired generally by an oral route. Some E coli are pathogenic and some may have a far more enhanced ability to colonise the human intestine than most others. Recently enterohaemorrhagic E coli have emerged. They can cause a number of intestinal illnesses in humans including bloody diarrhoea and haemolytic uraemic syndrome. These organisms produce a number of virulence factors particularly the Shiga-like toxins (verotoxins). The intestines of animals may be the reservoir of these organisms for human infection, and cattle particularly have been shown to harbour them. Food, especially undercooked meat products, have been associated with a number of outbreaks throughout the world. While a certain serotype O157.H7 has been associated with many outbreaks throughout the world, other serotypes, particularly 0111.H-, have also been reported. This latter serotype appears to be more common in Australia.  相似文献   

Resident forum abstracts
EVALUATION OF THROMBOELASTOGRAPHY (TEG) IN NORMAL CATS     

AJ Alwood  AB Downend  KA Slensky  JA Fox  SA Simpson  SM Donahue  LS Waddell  CM Otto 《Journal of Veterinary Emergency and Critical Care》2004,14(Z1):S1-S17

Objective: To establish normal parameters of thromboelastography (TEG) in healthy adult cats. Background: Thromboelastography (TEG) is an in vitro test of coagulation that has been shown to be useful in humans, dogs and select species to identify and quantify alterations of hemostasis (e.g., hypercoagulable and hypocoagulable states). It has also been demonstrated to be useful in monitoring effects of anticoagulant therapies. This test has not been evaluated in cats. Methods: Blood was collected from 25 clinically normal cats by venipuncture using a 21 gauge×3 1/2 inch butterfly catheter and syringe for medial saphenous or jugular venipuncture. A single 1.8 mL sample in 3.8% Sodium Citrate (9:1) was collected from each cat. Recalcified whole blood was analyzed 30 minutes following collection with the TEG® 5000 analyzer (Haemoscope, Niles, IL). Analysis temperature was 37.6°C. TEG parameters recorded included: R‐value (represents initial fibrin formation), K (time from R to standard fixed measure of clot firmness which represents contributions of platelets and fibrinogen), maximum amplitude (MA; represents absolute clot strength), and alpha angle (α; the slope of TEG tracing which represents rate of clot formation). The coagulation index (CI) was derived from the formula generated for humans to provide an overall assessment of whether the sample was hyper‐ or hypocoagulable. Results: Values for the 25 normal cat samples are reported as mean ±2 standard deviations. R=2.97; 1.23–4.72; K=1.54, 0.38–2.71; α=70.70, 57.76–83.65; MA=58.50, 45.26–71.74 and CI=2.27, 0.07–4.46. Compared to historical information obtained on normal dogs, cats have significantly shorter R and K and larger α, MA and CI. Conclusions: TEG does have reproducible performance when used to evaluate coagulation status in normal cats. Compared to dogs, normal cats favor a hypercoagulable state. Species‐specific normal values are necessary for interpretation of TEG results. This test bears potential value for use in future experimental and clinical work to investigate hemostasis in cats receiving anticoagulant therapies or in cats suffering from diseases such as cardiomyopathy which are thought to be associated with altered coagulation status.  相似文献